PeptidePlaza
The Free Peptide Reference

Peptide Reconstitution Calculator

Calculate concentration, dose volume, and syringe units for lyophilized peptides. Every step shown.

This is a reference calculator. Always verify your math against the Certificate of Analysis and reconstitution instructions for your specific peptide. Research use only.

1 Quick start

Select a peptide or preset protocol, enter your vial strength and water volume, then set your target dose. The calculator will show concentration, dose volume (mL and µL), and how many units to draw on your syringe.

Common protocols:
Typical: 0.25–2.4 mg SC once weekly (human RCT dosing); 2.4 mg is the approved obesity dose.

2 Results

MeasurementValueCalculation
Concentration2.50 mg/mL5 ÷ 2
Dose volume0.100 mL (100 µL)0.25 ÷ 2.50
Syringe units10.0 U0.100 × 100
Dose (micrograms)250 µg0.25 × 1000

4 Reference

4.1 Formulas

Concentration = Vial strength ÷ Water added
Dose volume (mL) = Target dose ÷ Concentration
Dose volume (µL) = Dose volume × 1000
Syringe units = Dose volume × Syringe total units

4.2 Syringe conversion guide

Syringe typeTotal volumeTotal unitsUnit size
Insulin syringe1 mL100 U0.01 mL per U
Insulin syringe0.5 mL50 U0.01 mL per U
Insulin syringe0.3 mL30 U0.01 mL per U

4.3 Storage best practices

  • Lyophilized: Room temperature, protected from light and moisture. Typically stable 2–3 years.
  • Reconstituted: Refrigerate 2–8°C. Bacteriostatic water preserves it. Stable 2–4 weeks depending on peptide.
  • Frozen: –20°C or below extends shelf life to 6–12 months, though freeze-thaw cycles may reduce stability.
  • Always check the Certificate of Analysis for peptide-specific storage data.
Research use only. Peptides sold as lyophilized research chemicals are not approved for human consumption. This calculator is a mathematical tool for laboratory protocol planning — it is not medical advice. Always verify your reconstitution against the Certificate of Analysis and the manufacturer's instructions.